Cell viability, apoptosis, intracellular reactive oxygen species (ROS), malondialdehyde (MDA), mitochondrial membrane potential (MMP) and caspase-3 activity were detected. Normal human liver cells ( HL- 7702) were treated with Cd(2+) for 21 h, and then co-treated with EGCG for 3 h. The objective of this study was to clarify the effect and detailed mechanism of EGCG on Cd(2+)-induced apoptosis. Nevertheless, the study of EGCG on Cd(2+)-induced apoptosis is rarely reported. Apoptosis is one of the key events in cadmium (Cd(2+))-induced cytotoxicity. Compounds 1, 2, 13, 14, and 19 exhibited weak inhibition of the cytotoxic effect of d-galactosamine on the human hepatic cell line HL- 7702.ĮGCG inhibits Cd(2+)-induced apoptosis through scavenging ROS rather than chelating Cd(2+) in HL- 7702 cells.Īn, Zhen Qi, Yongmei Huang, Dejun Gu, Xueyan Tian, Yihong Li, Ping Li, Hui Zhang, YingmeiĮpigallocatechin-3-gallat (EGCG), the major catechin in green tea, shows a potential protective effect against heavy metal toxicity to humans. Notably, the use of acetic acid-d4 was required to enable identification of the dibenzoyl glycoside structures.
Twelve new dibenzoyl derivatives sophodibenzoside A-L (1-12) and five new isoflavone glycosides (13-17) have been isolated from the roots of Sophora flavescens together with eight known compounds (18-25). Shen, Yi Feng, Zi-Ming Jiang, Jian-Shuang Yang, Ya-Nan Zhang, Pei-Cheng Dibenzoyl and isoflavonoid glycosides from Sophora flavescens: inhibition of the cytotoxic effect of D-galactosamine on human hepatocyte HL- 7702.
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